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What is an RT-PCR Machine?

An RT PCR machine is a laboratory instrument designed for the quantitative analysis of gene expression and the detection of RNA and DNA in biological samples. This machine, often referred to as an “RT-PCR machine,” utilizes the polymerase chain reaction (PCR) technique to amplify specific DNA or RNA sequences within a sample, allowing for the detection and quantification of target sequences.

Equipped with a thermal cycler, the RT-PCR machine performs a series of precise heating and cooling cycles to facilitate the amplification process. The resulting amplified sequences are then detected and quantified in real-time using fluorescent probes or dyes.

When considering the use of an RT-PCR machine, it is essential to factor in the “RT-PCR machine cost” and “RT-PCR machine price.” These costs can vary based on the machine’s features, brand, and the level of precision required for specific applications.

Best RT PCR Machine:

Choosing the best RT-PCR machine involves considering factors like sensitivity, specificity, throughput, ease of use, and price. Here are some of the top RT PCR machine available on the market. Brand like Thermofisher, Biorad, Roche are among the top manufacturer of RT PCR Machine:

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Company	Thermofisher Scientific	Thermofisher Scientific	Roche	Roche	Biorad
Item	Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System	Applied Biosystems™ QuantStudio™ 7 Pro Real-Time PCR System	LightCycler®480 Instrument II	LightCycler® 96 Instrument	C1000 Series Touch Thermal Cyclers
Reviews	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐
Catalog Number	A28573 / A28569 / A28138 / A28574 / A28139 / A28575	A43163 / A43170 / A43184 / A43162 / A43169 / A43183 / A43164 / A43171 / A43185	05015278001	05815916001	1851138 / 1851148 / 1851196 / 1851197
Description	QuantStudio 5: Superior performance, dye versatility, security options in affordable, user-friendly RT PCR system.	QuantStudio 7: Superior performance, dye versatility, security options in affordable, user-friendly real-time PCR system.	LightCycler® 480 II: Rapid, plate-based thermal cycler with integrated RT PCR detection, featuring improved Peltier-based technology in a silver block	Roche LightCycler® 96: Optimal mid-size, mid-throughput real-time PCR solution, delivering highest standards expected from LightCycler® Systems.	C1000 Touch thermal cycler with 96-deep well module: Run PCR reactions in plate or tube formats, with adjustable heated lid.
Applications	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene detection, Gene expression analysis, And genetic variation analysis, Multiplexing	Relative & absolute quantification, melting peak, endpoint genotyping for gene analysis.	Amplification/PCR, Cloning, Cycle sequencing, Gene expression studies, & Mutagenesis
Temprature Range	4–99.9°C	4–99.9°C	37 to 95 °C	37 to 98 °C	0 to 100°C
Display	Color touch screen	Color touch screen	Color touch screen	Color touch screen	Large touch screen
Wells	96 and 384 wells	96 and 384 wells	96 and 384 wells	96 wells	96, 384 and Dual 48/48 wells
Time	Inquire	Inquire	40 minutes (384-well PCR plate)	96 wells	Less than an hour.
Features	Compatible with automation	Compatible with automation	Customizable channels and filters; Interchangeable blocks	Connectivity (USB, Network); Silver block	Temperature gradient; Connectivity (USB storage)
Heating Rate	Maximum block ramp rate: 96-well 0.1 mL block: 9.0°C/sec 96-well 0.2 mL block: 6.5°C/sec 384-well block: 6.0°C/sec	Maximum block ramp rate: 96-well 0.1 mL block: 9.0°C/sec 96-well 0.2 mL block: 6.5°C/sec 384-well block: 6.0°C/sec	4.4 to 4.8 °C/sec	4.4 to 4.8 °C/sec	2.5°C/sec (Max)
Dimensions	(H x W x D) 40 x 27 x 50 cm	(H x W x D) 54.7 x 33.8 x 52.5 cm	24 in x 24 in x 21.5 in (57.4 cm x 58.8 cm x 49.7 cm)	(wXdXh) 40 x 40 x 53 cm (16 x 16 x 21 inches)	(WxDxH) 33 x 46 x 20 cm (13 x 18 x 8 inches)
Cooling Rate	Inquire	Inquire	96-well block: 2.2°C/s & 384-well block: 2.5°C/s	2.2°C/sec(Max)	2.5°C/sec (Max)
Volume	96-well 0.1 mL block: 10–30 µL 96-well 0.2 mL block: 10–100 µL 384-well block: 5–20 µL	96-well 0.1 mL block: 10–30 µL 96-well 0.2 mL block: 10–100 µL 384-well block: 5–20 µL	96-well PCR plate: 10 - 100 µl 384-well PCR plate: 5 - 20 µl	10-50 μl	Inquire
Temp uniformity	Inquire	Inquire	Inquire	Range (max-min) 0.4°C; STD	±0.4°C well-to-well within 10 sec of arrival at 90°C
Lid Type	Inquire	Inquire	Inquire	Inquire	Fully adjustable heated lid
Detection	Inquire	Inquire	Six detection filters; Cooled CCD camera	Cooled CCD camera; Up to 4 colors; Glass fiber optics	Inquire
Channels	Inquire	Inquire	6	4	Inquire
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Compare Top Rt pcr machine price

Header
Company	Thermofisher Scientific	Thermofisher Scientific	Roche	Roche	Biorad
Item	Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System	Applied Biosystems™ QuantStudio™ 7 Pro RT PCR System	LightCycler®480 Instrument II	LightCycler® 96 Instrument	C1000 Series Touch Thermal Cyclers
Reviews	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐	⭐⭐⭐⭐⭐
Catalog Number	A28573 / A28569 / A28138 / A28574 / A28139 / A28575	A43163 / A43170 / A43184 / A43162 / A43169 / A43183 / A43164 / A43171 / A43185	05015278001	05815916001	1851138 / 1851148 / 1851196 / 1851197
Description	QuantStudio 5: Superior performance, dye versatility, security options in affordable, user-friendly RT PCR system.	QuantStudio 7: Superior performance, dye versatility, security options in affordable, user-friendly real-time PCR system.	LightCycler® 480 II: Rapid, plate-based thermal cycler with integrated real-time detection, featuring improved Peltier-based technology in a silver block	Roche LightCycler® 96: Optimal mid-size, mid-throughput real-time PCR solution, delivering highest standards expected from LightCycler® Systems.	C1000 Touch thermal cycler with 96-deep well module: Run PCR reactions in plate or tube formats, with adjustable heated lid.
Applications	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene detection, Gene expression analysis, And genetic variation analysis, Multiplexing	Relative & absolute quantification, melting peak, endpoint genotyping for gene analysis.	Amplification/PCR, Cloning, Cycle sequencing, Gene expression studies, & Mutagenesis
Temperature Range	4–99.9°C	4–99.9°C	37 to 95 °C	37 to 98 °C	0 to 100°C
Display	Color touch screen	Color touch screen	Color touch screen	Color touch screen	Color touch screen
Price	$53,190	$64,760	$83,160	$31,880	$36,800
Power	Universal Power Supply	Universal Power Supply	Voltage: 220V to 240V	Voltage: 220V to 240V	Voltage: 120V
Warranty	12 months	12 months	12 months	12 months	12 months
Thermal Accuracy	±0.15°C	±0.15°C	±0.15°C	±0.15°C	±0.4°C
Ramp Rate	6.5°C/sec	6.5°C/sec	4.8°C/sec	4.8°C/sec	5°C/sec
Software	QuantStudio Design and Analysis Software	QuantStudio Design and Analysis Software	LightCycler® 480 Software	LightCycler® 96 Software	C1000 Software
Product Line	QuantStudio	QuantStudio	LightCycler	LightCycler	C1000 Series
Manufacturer	Applied Biosystems	Applied Biosystems	Roche	Roche	Biorad
Product Name	Applied Biosystems QuantStudio™ 5 Real-Time PCR System	Applied Biosystems QuantStudio™ 7 Pro Real-Time PCR System	LightCycler®480 Instrument II	LightCycler® 96 Instrument	C1000 Series Touch Thermal Cyclers
Applications	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene expression, genotyping, genetic variation analysis, microbial detection, pathogen detection	Gene expression, genetic variation analysis, and pathogen detection	Gene expression, genetic variation analysis, and pathogen detection	Gene expression, genetic variation analysis, and pathogen detection
Dimensions	36.3 x 39.3 x 54.9 cm	36.3 x 39.3 x 54.9 cm	28 x 39 x 35.5 cm	39.7 x 39 x 35.5 cm	19 x 18.1 x 44.7 cm
Weight	29.4 kg	29.4 kg	30 kg	26 kg	20 kg
Get Pricing	Get Pricing	Get Pricing	Get Pricing	Get Pricing	Get Pricing
Download Brochure	Download	Download	Download	Download	Download